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Within the boundaries of the Danube River Basin, several distinct ecoregions have been defined, based on the different geographical distributions of fauna and flora depending on the natural characteristics of the environment.

We Pass was an initiative aiming to facilitate fish migration in the Danube River Basin, set up by the ICPDR, Jaroslav Černi Institute, DDNI, CDM SMITH, OAK Consultants, and the Norwegian Institute for Nature Research. The focus was on the preservation and reestablishment of endangered fish species migration routes in the Danube River, its tributaries, and specifically at the Iron Gates.