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As both the largest remaining natural wetland and second largest river delta in Europe, the Danube Delta is one of the continent's most valuable habitats for wetland wildlife. Pollution and discharge manipulation from upstream has a huge effect on this area of high biodiversity, plus the delta continues to change ecologically itself – the Danube Delta spreads seaward at a rate of 10 to 25 metres every year.

The Sava is the Danube's largest tributary of the Danube in terms of discharge and the second largest in terms of catchment area. The Sava is shared by Slovenia, Croatia, Bosnia and Herzegovina, Serbia and Montenegro. The joint management arrangements acted as a pilot for the implementation of the European Union's Water Framework Directive for the Danube and Europe.

Covering an area of 157,186 km², the Tisza River Basin is the largest sub-basin of the Danube River Basin. The Tisza River is the longest tributary of the Danube (966 km), and second largest in terms of flow after the Sava. The countries of the Tisza Basin (Ukraine, Romania, Hungary, Slovakia, Serbia) agreed to close transboundary co-operation, aiming to achieve integrated water resources management of the Basin.

The Drava River is the fourth largest, as well as the fourth longest Danube tributary. It connects the Alps with the Danube and the Black Sea. The Drava has been considerably regulated with dams constructed to generate hydroelectricity and channels dredged to direct its flow. Nevertheless, natural habitats along the middle and lower reaches host unique varieties of flora and fauna, and several endemic species.

The Danube and its tributaries host a variety of fascinating and diverse ecological territories with many unique plants and animals. The rich and unique biodiversity and riverine habitats have been under severe pressure due to human activities for centuries. However, today the protection of environment and biodiversity is on the political agenda of the different Danubian States.

The Danube River Basin is Europe's second largest river basin, with a total area of 801,463 km². It is the world's most international river basin, flowing through the territory of 19 countries. The ecosystems of the Danube River Basin are highly valuable in environmental, economic, historical and social terms, but they are subject to increasing pressure and serious threats of pollution from agriculture, industry and cities.

Agriculture has long been a major source of income for many people living in the Danube River Basin. Today however, it is also a major source of pollutants – including fertilizers and pesticides and facing emerging issues like water scarcity and droughts.

The International Commission for the Protection of the Danube River (ICPDR) works to ensure the sustainable and equitable use of waters in the Danube River Basin. The work of the ICPDR is based on the Danube River Protection Convention (DRPC), the major legal instrument for cooperation and transboundary water management in the Danube River Basin.

The Black Sea Commission is an intergovernmental organisation responsible for the implementation of the Convention on the Protection of the Black Sea against Pollution, and sets the legal framework required for regional cooperation and the activities necessary to reduce pollution and enhance the protection of the marine environment.

The territory of Bulgaria is 110,912 km². The country is characterized by a rich diversity in morphological, geological, geomorphological, hydro-climatic and soil-biogenic aspects.