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Sub-basins are smaller working units within a larger river basin or catchment area. They represent a distinct geographical area that is delineated based on the natural drainage patterns of rivers and their tributaries. Sub-basins are used to facilitate the management and assessment of water resources, allowing for more focused and localized analysis and planning within the broader context of the overall river basin.

A recent large-scale modelling study has shown that restoring floodplains and reconnecting cut-off water bodies with the Danube River, and its tributaries could play a significant role in improving water quality and removing nitrate pollution in the Danube River Basin.