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Covering an area of 157,186 km², the Tisza River Basin is the largest sub-basin of the Danube River Basin. The Tisza River is the longest tributary of the Danube (966 km), and second largest in terms of flow after the Sava. The countries of the Tisza Basin (Ukraine, Romania, Hungary, Slovakia, Serbia) agreed to close transboundary co-operation, aiming to achieve integrated water resources management of the Basin.

The Drava River is the fourth largest, as well as the fourth longest Danube tributary. It connects the Alps with the Danube and the Black Sea. The Drava has been considerably regulated with dams constructed to generate hydroelectricity and channels dredged to direct its flow. Nevertheless, natural habitats along the middle and lower reaches host unique varieties of flora and fauna, and several endemic species.

The status of waters of the Danube River Basin is largely influenced by inputs of pollutants, particularly organic material, nutrients, hazardous substances and plastics. The ICPDR regularly updates specific emission inventories addressing both point source and diffuse emissions. The ICPDR also develops policy guidance documents providing recommendations for Danube countries on how to address pollution problems and the measures that need to be implemented in order to reduce emissions. In addition, the ICPDR supports capacity building activities on issues related to pollution control.