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Three sub-basins of the Danube are partly located in Ukraine - the Tisza, Prut and Siret basins, as well as part of the Danube Delta. Furthermore, 2.7 million people live in the Ukrainian part of the Danube Basin, which is 3.3% of the total Danube Basin District. Ukraine has been a Signatory State to the Danube River Protection Convention since 1994. The Convention was ratified by the Ukrainian Parliament in 2002.

The floodplains and wetlands of the Danube basin are uniquely valuable ecosystems in global terms, although few areas are still in their natural or near-natural state.

As both the largest remaining natural wetland and second largest river delta in Europe, the Danube Delta is one of the continent's most valuable habitats for wetland wildlife. Pollution and discharge manipulation from upstream has a huge effect on this area of high biodiversity, plus the delta continues to change ecologically itself – the Danube Delta spreads seaward at a rate of 10 to 25 metres every year.

Covering an area of 157,186 km², the Tisza River Basin is the largest sub-basin of the Danube River Basin. The Tisza River is the longest tributary of the Danube (966 km), and second largest in terms of flow after the Sava. The countries of the Tisza Basin (Ukraine, Romania, Hungary, Slovakia, Serbia) agreed to close transboundary co-operation, aiming to achieve integrated water resources management of the Basin.

Since the 16th century, people have been changing the natural course of the rivers in the Danube River Basin, mainly for flood defence, hydropower generation and navigation. All these changes affect the ecological quality of the rivers. Changes in the depth or width of a river typically reduce flow rates, interrupting natural sediment transportation as well as the migration routes of animals.