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The Republic of Serbia covers an area of 88,499 km² and includes two provinces: Vojvodina (21,614 km²) and Kosovo and Metohija (10,910 km²); the latter being currently under an international protectorate. Approximately 92% of the country lies within the Danube Basin (accounting for 10% of the total Basin). Of this land, 30% is forested. With more than 90% of Serbia’s renewable water resources originating from outside national territory, international cooperation on water issues is crucial for sustainable water management.

The Sava is the Danube's largest tributary of the Danube in terms of discharge and the second largest in terms of catchment area. The Sava is shared by Slovenia, Croatia, Bosnia and Herzegovina, Serbia and Montenegro. The joint management arrangements acted as a pilot for the implementation of the European Union's Water Framework Directive for the Danube and Europe.

Covering an area of 157,186 km², the Tisza River Basin is the largest sub-basin of the Danube River Basin. The Tisza River is the longest tributary of the Danube (966 km), and second largest in terms of flow after the Sava. The countries of the Tisza Basin (Ukraine, Romania, Hungary, Slovakia, Serbia) agreed to close transboundary co-operation, aiming to achieve integrated water resources management of the Basin.

The Danube River Basin is Europe's second largest river basin, with a total area of 801,463 km². It is the world's most international river basin, flowing through the territory of 19 countries. The ecosystems of the Danube River Basin are highly valuable in environmental, economic, historical and social terms, but they are subject to increasing pressure and serious threats of pollution from agriculture, industry and cities.