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Three sub-basins of the Danube are partly located in Ukraine - the Tisza, Prut and Siret basins, as well as part of the Danube Delta. Furthermore, 2.7 million people live in the Ukrainian part of the Danube Basin, which is 3.3% of the total Danube Basin District. Ukraine has been a Signatory State to the Danube River Protection Convention since 1994. The Convention was ratified by the Ukrainian Parliament in 2002.

The Republic of Serbia covers an area of 88,499 km². Approximately 92% of the country lies within the Danube Basin (accounting for 10% of the total basin). Of this land, 30% is forested. With more than 90% of Serbia’s renewable water resources originating from outside national territory, international cooperation on water issues is crucial for sustainable water management.

As both the largest remaining natural wetland and second largest river delta in Europe, the Danube Delta is one of the continent's most valuable habitats for wetland wildlife. Pollution and discharge manipulation from upstream has a huge effect on this area of high biodiversity, plus the delta continues to change ecologically itself – the Danube Delta spreads seaward at a rate of 10 to 25 metres every year.

The Sava is the Danube's largest tributary of the Danube in terms of discharge and the second largest in terms of catchment area. The Sava is shared by Slovenia, Croatia, Bosnia and Herzegovina, Serbia and Montenegro. The joint management arrangements acted as a pilot for the implementation of the European Union's Water Framework Directive for the Danube and Europe.