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The Danube is very significant to Romania, since the country is located almost entirely within the Danube River Basin. The Romanian section covers almost a third of the surface area of the Basin, and over a third of the river’s length flows through the country. Crucially, the Romanian (and also Ukrainian) Danube is the end carrier of all wastewater discharges into the Black Sea.

The Danube begins at the confluence of the Brigach and Breg Rivers in the city of Donaueschingen located in the Black Forest, Baden-Württemberg. The German Danube catchment area covers an area of about 56,200 km²; its share of the total Danube catchment area is approx. 7%. The German Danube basin is bordered by the Rhine basin to the west and the Elbe River basin to the east.

As both the largest remaining natural wetland and second largest river delta in Europe, the Danube Delta is one of the continent's most valuable habitats for wetland wildlife. Pollution and discharge manipulation from upstream has a huge effect on this area of high biodiversity, plus the delta continues to change ecologically itself – the Danube Delta spreads seaward at a rate of 10 to 25 metres every year.

Covering an area of 157,186 km², the Tisza River Basin is the largest sub-basin of the Danube River Basin. The Tisza River is the longest tributary of the Danube (966 km), and second largest in terms of flow after the Sava. The countries of the Tisza Basin (Ukraine, Romania, Hungary, Slovakia, Serbia) agreed to close transboundary co-operation, aiming to achieve integrated water resources management of the Basin.