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Switzerland, officially the Swiss Confederation, is a landlocked country located at the convergence of Western, Central Southern Europe. Switzerland's natural landscape is marked by its numerous lakes and mountains, geographically divided among the Swiss Plateau, the Alps, and the Jura.

The Czech Republic is situated at the roof of Europe and its waters are drained through rivers into three different seas. The majority of the territory is drained by the Elbe and Oder to the North and Baltic Seas. The Morava River drains almost 28 % of the country to the Danube. Nearly 2.8 million inhabitants – around 3.46 % of the population in the Danube River Basin – live in this area.

Organic pollution has been on the rise in the Danube over the last century, as human activities have resulted in increasing loads of wastewater rich in organic matter. The most serious organic pollution problems occur in tributaries that regularly receive untreated or inadequately treated wastewater from industrial plants and municipalities. Thanks to the significant improvement of wastewater infrastructure and services in the Danube countries, the basin-wide organic emissions reduced by 60% since the mid 2000s.