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Evidence on plastic pollution has already been gathered in both the freshwater systems and the marine environment (including the Danube and the Black Sea) over the last decades. Scientific investigations identified strong linkages between marine pollution, terrestrial areas and land-based activities. Poor waste management, everyday littering, plastic industry facilities, consuming of products of textile and cosmetic industries in households and tire abrasion pollute rivers that further discharge plastic litter into the receiving seas. While priority should be given to reducing plastic pollution at source, river clean-up actions are also highly important to eliminate plastic litter accumulation hot-spots.

The Danube River is seeing an influx of new non-native species, stemming from the increasing interconnections with various European and global water bodies via canals and other waterways designed to facilitate navigation. It is currently believed that alien species are very likely to become even more significant in the future, as the importance of the Danube as an international waterway increases.