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The Republic of Serbia covers an area of 88,499 km² and includes two provinces: Vojvodina (21,614 km²) and Kosovo and Metohija (10,910 km²); the latter being currently under an international protectorate. Approximately 92% of the country lies within the Danube Basin (accounting for 10% of the total Basin). Of this land, 30% is forested. With more than 90% of Serbia’s renewable water resources originating from outside national territory, international cooperation on water issues is crucial for sustainable water management.

The Danube is very significant to Romania, since the country is located almost entirely within the Danube River Basin. The Romanian section covers almost a third of the surface area of the Basin, and over a third of the river’s length flows through the country. Crucially, the Romanian (and also Ukrainian) Danube is the end carrier of all wastewater discharges into the Black Sea.

As both the largest remaining natural wetland and second largest river delta in Europe, the Danube Delta is one of the continent's most valuable habitats for wetland wildlife. Pollution and discharge manipulation from upstream has a huge effect on this area of high biodiversity, plus the delta continues to change ecologically itself – the Danube Delta spreads seaward at a rate of 10 to 25 metres every year.

The Sava is the Danube's largest tributary of the Danube in terms of discharge and the second largest in terms of catchment area. The Sava is shared by Slovenia, Croatia, Bosnia and Herzegovina, Serbia and Montenegro. The joint management arrangements acted as a pilot for the implementation of the European Union's Water Framework Directive for the Danube and Europe.

Covering an area of 157,186 km², the Tisza River Basin is the largest sub-basin of the Danube River Basin. The Tisza River is the longest tributary of the Danube (966 km), and second largest in terms of flow after the Sava. The countries of the Tisza Basin (Ukraine, Romania, Hungary, Slovakia, Serbia) agreed to close transboundary co-operation, aiming to achieve integrated water resources management of the Basin.